Live cell imaging

Live cell imaging

Live cell imaging using microscope and live cell chamber has been widely
focused research in cell biology. To understand the functional integrity of a
whole living organism, it is important to first have a clear understanding of the
interactions between both individual cells and single molecules within these cells.

Using the latest in biomolecular/cellular imaging technology, researchers are
focusing on investigating such biological interactions at both the molecular and
cellular levels. This involves direct imaging of the “presence” of single molecules
and individual cells as well as the use of real-time imaging of dynamic,
reversible biological signaling “events” such as protein-protein interaction, protein
modification and intracellular calcium changes.

Moreover, this imaging technology can be combined with protein manipulation techniques
to elucidate the functional contribution of these dynamic interactions and signal
transduction events in the whole living organism.

Incubator system for live cell imaging
Live Cell Instrument (LCI) is an innovative company that designs, develops and
manufactures live-cell microscopy environmental control systems for qualitative and or
quantitative microscopy. Our products are dedicated to the science of live-cell microscopy
and include imaging systems, micro-observation environmental control instruments, and
specialized fittings and adapters as well as numerous supplemental items.
  • Temperature
    Most mammalian cells grow at 35-37oC. If the temperature is not optimally controlled, cell growth can be largely compromised.
    High temperature is even more detrimental to cells since it results in immediate cell death. Therefore, maintaining the temperature within an
    optimal range is very important for cellular imaging. When imaging with a high numeric aperture lens it is necessary to control the temperature of the
    objective also, as the optical coupling of the medium (oil, glycerin or water) acts as a thermal coupling medium, drawing heat away from the specimen.
  • pH
    Correct pH is crucial for cultured cells to maintain their normal physiological states and thus remain viable over the long-term. The pH of the medium should be
    maintained around pH 7.4. In a bicarbonate buffer system, which is the most widely used buffer system in cell culture, pH depends on the level of CO2.
    Thus, the CO2 level should be maintained during cell imaging.
  • Humidity
    All incubator systems should maintain internal humidity to prevent medium evaporation. Evaporation causes the accumulation of cell metabolites and medium
    constituents that will eventually impair cell growth. Furthermore, certain osmolarities enables cell growth well. (e.g. Mammalian cells grow well at osmolarities
    between 260 and 320 mOsm.)
  • Oxygen
    Adequate oxygen level is necessary to culture and image the live cell in incubator. Typical cells require oxygen for respiration in vivo, and also oxygen can reduce
    the photo-damage and bleaching during fluorescence illumination.
  • Lens warming
    When the use of high numeric aperture lenses for high-resolution imaging is required, it is necessary to control the temperature of the objectives due to
    temperature gradients between the specimen and the front lens elements. Since the optical coupling medium (oil, glycerin, or water) acts as a thermal coupling
    medium, it draws heat away from the specimen.

Advanced technologies of microscope and live cell incubator enables imaging of live specimens for long time and designing challenge of biological research. Imaging of various cells has been
reported throughout the continued development of microscope and live cell incubator. In case of live cell incubator, promising technique and current instrument has potential to form perfect
condition for various living cells. Live Cell Instrument as a leading company in live cell incubator currently develop new technology to overcome the remained bottle-necks and provide perfect
solution for live cell imaging.